Welcome to Biological Science Advanced Studies. This site is designed for biology students of A-level, AS and A2, covering the related topics in Biology and Human Biology.
Too complicated, the book is much easier just 3 steps:
1-isolate mrna from cells.
2-add reverse transcriptase to make single strand cdna, add dna polymerase to make it double stranded.
3-add dna to plasmid and insert in e.coli cells.
-a gene library is a set of thousands of DNA fragments from one organism
- DNA of organism is cut using a restriction endonuclease
- each fragment is inserted into plasmid to make a recombinant plasmid
-recombinant plasmids are then inserted in bacterial cells
-mRNA is isolated from cells
-reverse transcriptase is used to make a DNA copy of the mRNA
-use DNA polymerase to make the single stranded cDNA double stranded
- each piece of DNA is added to a seperate plasmid and inserted into a bacterial cell
Too complicated, the book is much easier just 3 steps:
1-isolate mrna from cells.
2-add reverse transcriptase to make single strand cdna, add dna polymerase to make it double stranded.
3-add dna to plasmid and insert in e.coli cells.